Case Study Overview:
The standard solution to co-elution of native host cell proteins (HCPs) typically involves adjustments to downstream purification conditions.
In co-elution cases, an HCP is bound to the product protein. However, even highly specific affinity capture cannot separate the product from impurity. Instead, the HCP impurity must be degraded before purification, or its association must be disrupted.
The Challenge:
Our team of experts set out to find a way to avoid the expensive, time-consuming, complex downstream development work and customization of the purification process needed in these situations.
The Results:
We solved this problem in a timely and cost-effective manner using cell line engineering. The strategy resulted in the removal of the specific host cell protein, which additionally led to an improvement in product titer, perhaps due to the decreased strain on the cells’ production capacity with the removal of the host cell protein.
Access our complete white paper on the topic to explore the complete technical work and learn how we accomplished this important task during cell line development.
